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KMID : 0903520040470020258
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2004 Volume.47 No. 2 p.258 ~ p.264
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A Study on Hypoglycemic Effects of Crude Extracts of Schizandrae Fructus
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Ko Byoung-Seob
Park Seong-Kyu
Choi Soo-Bong
Jun Dong-Wha
Choi Mi-Kyung
Park Sun-Min
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Abstract
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Hypoglycemic effect of Schizandrae Fructus (SF) extract containing in Okchun-san was determined on 3T3-L1 fibroblasts and adipocytes by investigating insulin-like activity, insulin sensitizing activity and α-glucoamylase suppressing activity. SF were extracted by using 70% ethanol followed by XAD-4 column chromatography with a mixture solvent of methanol and water, and the fractional extractions were utilized for assaying hypoglycemic effect. No inhibition of α-glucoamylase activity of SF was observed. Insulin-like activity in 3T3-L1 adipocytes was not shown by SF. A significant insulin sensitizing activity of SF extractions was observed in 3T3-L1 adipocytes, giving SF extractions with 1 ng/ml insulin to reach glucose uptake level increased by 50 ng/ml of insulin alone. When cells were treated with SF (Fr. 4 or 5) plus 1 ng/ml insulin, glucose uptake was increased more than seven times as compared to 1 ng/ml of insulin alone, suggesting that SF extracts increased GLUT4 content by enhancing insulin signaling. These data suggest that SF extracts (especially Fr. 4 and 5) contains an effective insulin sensitizing compounds for hypoglycemic activity in 3T3-L1 adipocytes.
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KEYWORD
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insulin sensitizer, insulin-like substance, a-glucoamylase, Okchun-san(OCH), 3T3-L1 adipocytes
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